mercoledì 17 luglio 2019

(Pioppo) Assorbimento di ftalato diottico di populus alba diottile da acqua contaminata

Assorbimento di ftalato diottico di populus alba diottile da acqua contaminata

Autori e affiliazioni
Francesca VannucchiAlessandra FranciniEmail autoreErika C. PierattiniAndrea RaffaelliLuca Sebastiani
ricerca articolo
Primo online: 02 luglio 2019
Gli ftalati sono micro-inquinanti di grande preoccupazione a causa dei loro effetti negativi sul funzionamento degli ecosistemi e sulla salute umana. Grazie alla sua capacità di assorbimento e accumulo di inquinanti organici, il clone di Populus alba L. "Villafranca" potrebbe essere un buon candidato per ridurre gli impatti derivati ​​dalla persistenza di tali composti nell'ambiente. Abbiamo studiato la risposta e l'assorbimento di dioctyl phthalate (DOP) da parte del pioppo, coltivato in condizioni idroponiche, per 21 giorni con 0, 40 e 400 μg L-1 di d4-DOP. Le piante trattate, dopo 21 giorni di 400 μg di L-1 d4-DOP, hanno mostrato un aumento della biomassa secca delle radici (+ 29%) a scapito delle parti aeree (-8%) rispetto al controllo. Lo sviluppo della radice potrebbe essere sostenuto dall'aumento dell'uptake di Mg da parte del pioppo. L'analisi LC-MS / MS ha dimostrato l'assorbimento e l'accumulo nelle radici di d4-DOP a partire dal primo giorno (rispettivamente 3,5 ± 3,29 e 7,1 ± 3,28 in 40 e 400 μg L-1 d4-DOP), nonostante la volatilizzazione di d4-DOP fosse osservato dalla soluzione nutritiva. L'interazione chimica tra d4-DOP e Zn si è verificata nelle radici di piante trattate con l'alta concentrazione di d4-DOP, senza limitare la concentrazione di Zn nelle foglie. I risultati confermano l'elevata tolleranza del clone "Villafranca" allo xenobiotico e suggeriscono la capacità del pioppo nell'assorbimento e accumulo di d4-DOP a livello della radice.

Populus alba dioctyl phthalate uptake from contaminated water

  • Francesca Vannucchi
  • Alessandra FranciniEmail author
  • Erika C. Pierattini
  • Andrea Raffaelli
  • Luca Sebastiani
  1. 1.
  2. 2.
Research Article


Phthalates are micro-pollutants of great concern due to their negative effects on ecosystem functioning and human health. Thanks to its capability in uptake and accumulation of organic pollutants, Populus alba L. “Villafranca” clone could be a good candidate for reducing the impacts derived by the persistence of such compounds in the environment. We investigated plant response and uptake of dioctyl phthalate (DOP) by poplar, grown in hydroponics condition, for 21 days with 0, 40, and 400 μg L−1 of d4-DOP. Treated plants, after 21 days of 400 μg L−1 d4-DOP, showed an increase in root dry biomass (+ 29%) at the expense of aerial parts (− 8%) compared with control. The root development could be sustained by the increase of Mg uptake by poplar. LC-MS/MS analysis demonstrated the uptake and accumulation in roots of d4-DOP starting from day one (3.5 ± 3.29 and 7.1 ± 3.28 in 40 and 400 μg L−1 d4-DOP respectively), despite volatilization of d4-DOP was observed from nutritive solution. The chemical interaction between d4-DOP and Zn occurred in roots of plants treated with the high d4-DOP concentration, without limiting the Zn concentration in leaves. Results confirm the high tolerance of “Villafranca” clone to xenobiotic and suggest the poplar capability in d4-DOP uptake and accumulation at root level.


DOP Micro-pollutants Phthalates Poplar Nutrients Volatility 



  1. Abdel daiem MM, Rivera-Utrilla J, Ocampo-Pérez R, Méndez-Díaz JD, Sánchez-Polo M (2012) Environmental impact of phthalic acid esters and their removal from water and sediments by different technologies–a review. J Environ Manag 109:164–178CrossRefGoogle Scholar
  2. Arnon DI, Hoagland DR (1940) Crop production in artificial culture solutions and in soils with special reference to factors influencing yields and absorption of inorganic nutrients. Soil Sci 50:463–485Google Scholar
  3. Baca SG (2013) Zinc(II) complexes based on ortho-phthalic acid and ancillary N-donor ligands. ChemInform.
  4. Baca SG, Filippova IG, Gherco OA, Gdaniec M, Simonov YA, Gerbeleu NV, Franz P, Basler R, Decurtins S (2004) Nickel (II)-, cobalt (II)-, copper (II)-, and zinc (II)-phthalate and 1-methylimidazole coordination compounds: synthesis, crystal structures and magnetic properties. Inorg Chim Acta 357(12):3419–3429CrossRefGoogle Scholar
  5. Briggs GG, Bromilow RH, Evans AA (1982) Relationships between lipophilicity and root uptake and translocation of non-ionized chemicals by barley. Pestic Sci 13:495–504CrossRefGoogle Scholar
  6. Cai QY, Mo CH, Zeng QY, Wu QT, Férard JF, Antizar-Ladislao B (2008) Potential of Ipomoea aquatica cultivars in phytoremediation of soils contaminated with di-n-butyl phthalate. Environ Exp Bot 62(3):205–211CrossRefGoogle Scholar
  7. Chen WC, Huang HC, Wang YS, Yen JH (2011) Effect of benzyl butyl phthalate on physiology and proteome characterization of water celery (Ipomoea aquatica Forsk.). Ecotoxicol Environ Saf 74(5):1325–1330CrossRefGoogle Scholar
  8. Chen HL, Yao J, Wang F (2013) Soil microbial and enzyme properties as affected by long-term exposure to phthalate esters. Adv Mater Res 726:3653–3656CrossRefGoogle Scholar
  9. Chi J (2009) Phthalate acid esters in Potamogeton crispus L. from Haihe River China. Chemosphere 77(1):48–52CrossRefGoogle Scholar
  10. Clara M, Windhofer G, Hartl W, Braun K, Simon M, Gans O, Scheffknecht C, Chovanec A (2010) Occurrence of phthalates in surface runoff, untreated and treated wastewater and fate during wastewater treatment. Chemosphere 78(9):1078–1084CrossRefGoogle Scholar
  11. Fu X, Du Q (2011) Uptake of di-(2-ethylhexyl) phthalate of vegetables from plastic film greenhouses. J Agric Food Chem 59(21):11585–11588CrossRefGoogle Scholar
  12. Gani KM, Tyagi VK, Kazmi AA (2017) Occurrence of phthalates in aquatic environment and their removal during wastewater treatment processes: a review. Environ Sci Pollut Res Int 24(21):17267–17284CrossRefGoogle Scholar
  13. Gao D, Li Z, Wen Z, Ren N (2014) Occurrence and fate of phthalate esters in full-scale domestic wastewater treatment plants and their impact on receiving waters along the Songhua River in China. Chemosphere 95:24–32CrossRefGoogle Scholar
  14. Gao M, Qi Y, Song W, Xu H (2016) Effects of di-n-butyl phthalate and di (e-ethylhexyl) phthlate on the growth, photosynthesis, and chlorophyll fluorescence of wheat seedlings. Chemosphere 151:76–83CrossRefGoogle Scholar
  15. Giachetti G, Sebastiani L (2006) Metal accumulation in poplar plant grown with industrial wastes. Chemosphere 64(3):446–454CrossRefGoogle Scholar
  16. Hafsi C, Debez A, Abdelly C (2014) Potassium deficiency in plants: effects and signaling cascades. Acta Physiol Plant 36(5):1055–1070CrossRefGoogle Scholar
  17. He L, Gielen G, Bolan NS, Zhang X, Qin H, Huang H, Wang H (2015) Contamination and remediation of phthalic acid esters in agricultural soils in China: a review. Agron Sustain Dev 35(2):519–534CrossRefGoogle Scholar
  18. Hermans C, Vuylsteke M, Coppens F, Cristescu SM, Harren FJ, Inzé D, Verbruggen N (2010) Systems analysis of the responses to long-term magnesium deficiency and restoration in Arabidopsis thaliana. New Phytol 187(1):132–144CrossRefGoogle Scholar
  19. Iori V, Pietrini F, Zacchini M (2012) Assessment of ibuprofen tolerance and removal capability in Populus nigra L. by in vitro culture. J Hazard Mater 229-230:217–223CrossRefGoogle Scholar
  20. Katsikantami I, Sifakis S, Tzatzarakis MN, Vakonaki E, Kalantzi OI, Tsatsakis AM, Rizos AK (2016) A global assessment of phthalates burden and related links to health effects. Environ Int 97:212–236CrossRefGoogle Scholar
  21. Krell HW, Sandermann H (1984) Plant biochemistry of xenobiotics purification and properties of a wheat esterase hydrolyzing the plasticizer chemical, bis(2-ethylhexy1)phthalate. Eur J Blochem 143(1):57–62CrossRefGoogle Scholar
  22. Li C, Chen J, Wang J, Han P, Luan Y, Ma X, Lu A (2016) Phthalate esters in soil, plastic film, and vegetable from greenhouse vegetable production bases in Beijing, China: concentrations, sources, and risk assessment. Sci Total Environ 568:1037–1043CrossRefGoogle Scholar
  23. Liang DW, Zhang T, Fang HH, He J (2008) Phthalates biodegradation in the environment. Appl Microbiol Biotechnol 80(2):183–198CrossRefGoogle Scholar
  24. Liao CS, Yen JH, Wang YS (2006) Effects of endocrine disruptor di-n-butyl phthalate on the growth of Bok choy (Brassica rapa subsp. chinensis). Chemosphere 65:1715–1722CrossRefGoogle Scholar
  25. Lloyd G, McCown B (1980) Commercially-feasible micropropagation of mountain laurel, Kolmialatifolia, by use of shoot tip culture. Combined Proceedings of International Plant Propagators’ Society 30:421–427Google Scholar
  26. Luo Y, Guo W, HaoNgo H, DucNghiem L, IbneyHai F, Zhang J, Liang S, Wang XC (2014) A review on the occurrence of micropollutants in the aquatic environment and their fate and removal during wastewater treatment. Sci Total Environ 473:619–641CrossRefGoogle Scholar
  27. Ma T, Luo Y, Christie P, Teng Y, Liu W (2012) Removal of phthalic esters from contaminated soil using different cropping systems: a field study. Eur J Soil Biol 50:76–82CrossRefGoogle Scholar
  28. Ma T, Christie P, Teng Y, Luo Y (2013) Rape (Brassica chinensis L.) seed germination, seedling growth, and physiology in soil polluted with di-n-butyl phthalate and bis (2-ethylhexyl) phthalate. Environ Sci Pollut Res 20(8):5289–5298CrossRefGoogle Scholar
  29. Magdouli S, Daghrir R, Brar SK, Drogui P, Tyagi RD (2013) Di 2-ethylhexylphtalate in the aquatic and terrestrial environment: a critical review. Environ Manag 127:36–49Google Scholar
  30. Markert B (1992) Presence and significance of naturally occurring chemical elements of the periodic system in the plant organism and consequences for future investigations on inorganic environmental chemistry in ecosystems. Vegetatio 103(1):1–30Google Scholar
  31. Marmiroli M, Pietrini F, Maestri E, Zacchini M, Marmiroli N, Massacci A (2011) Growth, physiological and molecular traits in Salicaceae trees investigated for phytoremediation of heavy metals and organics. Tree Physiol 31(12):1319–1334CrossRefGoogle Scholar
  32. Martínez-Hernández V, Leal M, Meffe R, De Miguel Á, Alonso-Alonso C, De Bustamante I et al (2018) Removal of emerging organic contaminants in a poplar vegetation filter. J Hazard Mater 342:482–491CrossRefGoogle Scholar
  33. Miller EL, Nason SL, Karthikeyan KG, Pedersen JA (2016) Root uptake of pharmaceuticals and personal care product ingredients. Environ Sci Technol 50:525–541CrossRefGoogle Scholar
  34. Net S, Sempéré R, Delmont A, Paluselli A, Ouddane B (2015) Occurrence, fate, behavior and ecotoxicological state of phthalates in different environmental matrices. Environ Sci Technol 49(7):4019–4035CrossRefGoogle Scholar
  35. Niu Y, Jin G, Zhang YS (2014) Root development under control of magnesium availability. Plant Signal Behav 9(9):e29720CrossRefGoogle Scholar
  36. Oca ML, Rubio L, Sarabia LA, Ortiz MC (2016) Dealing with the ubiquity of phthalates in the laboratory when determining plasticizers by gas chromatography/mass spectrometry and PARAFAC. J Chromatogr A 1464:124–140CrossRefGoogle Scholar
  37. Pierattini EC, Francini A, Raffaelli A, Sebastiani L (2016a) Degradation of exogenous caffeine by Populus alba and its effects on endogenous caffeine metabolism. Environ Sci Pollut Res 23(8):7298–7307CrossRefGoogle Scholar
  38. Pierattini EC, Francini A, Raffaelli A, Sebastiani L (2016b) Morpho-physiological response of Populus alba to erythromycin: a timeline of the health status of the plant. Sci Total Environ 569–570:540–547CrossRefGoogle Scholar
  39. Pierattini EC, Francini A, Huber C, Sebastiani L, Schröder P (2018) Poplar and diclofenac pollution: a focus on physiology, oxidative stress and uptake in plant organs. Sci Total Environ 636:944–952CrossRefGoogle Scholar
  40. Pilipović A, Orlović S, Rončević S, Nikolić N, Župunski M, Spasojević J (2015) Results of selection of poplars and willows for water and sediment phytoremediation. Agricult Forest 61:205–211Google Scholar
  41. Potters G, Pasternak TP, Guisez Y, Palme KJ, Jansen MA (2007) Stress-induced morphogenic responses: growing out of trouble? Trends Plant Sci 12(3):98–105CrossRefGoogle Scholar
  42. Romè C, Romeo S, Francini A, Andreucci A, Sebastiani L (2016a) Leaves position in Populus alba Villafranca clone reveals a strategy towards cadmium uptake response. Plant Growth Regul 79(3):355–366CrossRefGoogle Scholar
  43. Romè C, Huang XY, Danku J, Salt DE, Sebastiani L (2016b) Expression of specific genes involved in Cd uptake, translocation, vacuolar compartmentalization and recycling in Populus alba Villafranca clone. Indian J Plant Physiol 202:83–91CrossRefGoogle Scholar
  44. Romeh AA (2013) Diethyl phthalate and dioctyl phthalate in Plantago major L. Afr J Agric Res 8(32):4360–4364Google Scholar
  45. Romeo S, Francini A, Ariani A, Sebastiani L (2014) Phytoremediation of Zn: identify the diverging resistance, uptake and biomass production behaviours of poplar clones under high zinc stress. Water Air Soil Pollut 225:1813–1819CrossRefGoogle Scholar
  46. Romeo S, Francini A, Sebastiani L, Morabito D (2017) High Zn concentration does not impair biomass, cutting radial growth, and photosynthetic activity traits in Populus albaL. J Soils Sediments 17(5):1394–1402CrossRefGoogle Scholar
  47. Sandermann H (1994) Higher plant metabolism of xenobiotics: the ‘green liver’ concept. Pharmacogenetics 4(5):225–241CrossRefGoogle Scholar
  48. Schröder P, Navarro-Aviñó J, Azaizeh H, Goldhirsh AG, DiGregorio S, Komives T, Langergraber G, Lenz A, Maestri E, Memon AR, Ranalli A, Sebastiani L, Smrcek S, Vanek T, Vuilleumier S, Wissing F (2007) Using phytoremediation technologies to upgrade waste water treatment in Europe. Environ Sci Pollut Res Int 14(7):490–497CrossRefGoogle Scholar
  49. Stasinakis AS (2012) Review on the fate of emerging contaminants during sludge anaerobic digestion. Bioresour Technol 121:432–440CrossRefGoogle Scholar
  50. Sun J, Wu X, Gan J (2015) Uptake and metabolism of phthalate esters by edible plants. Environ Sci Technol 49:8471–8478CrossRefGoogle Scholar
  51. Vaz JLL, Duc G, Petit-Ramel M, Faure R, Vittori O (1996) Cd (II) complexes with phthalic acids: solution study and crystal structure of cadmium (II) phthalate hydrate. Can J Chem 74(3):359–364CrossRefGoogle Scholar
  52. Visscher AM, Paul AL, Kirst M, Guy CL, Schuerger AC, Ferl RJ (2010) Growth performance and root transcriptome remodeling of Arabidopsis in response to Mars-like levels of magnesium sulfate. PLoS One 5:e12348CrossRefGoogle Scholar
  53. Wu Z, Zhang X, Wu X, Shen G, Du Q, Mo C (2013) Uptake of di (2-ethylhexyl) phthalate (DEHP) by the plant Benincasa hispida and its use for lowering DEHP content of intercropped vegetables. J Agric Food Chem 61(22):5220–5225CrossRefGoogle Scholar
  54. Zavoda J, Cutright T, Szpak J, Fallon E (2001) Uptake, selectivity, and inhibition of hydroponic treatment of contaminants. J Environ Eng 127(6):502–508CrossRefGoogle Scholar
  55. Zeng QY, Mo CH, Cai QY, Mo C, Wen R (2010) Root morphological and physiological characteristics of two genetypes of Brassica parachinensis and their effect on di (2-ethylhexil) phtalate (DEHP) uptake. Acta Sci Vet 30:1280–1285Google Scholar
  56. Zhang Y, Zhang H, Sun X, Wang L, Du N, Tao Y, Sun G, Erinle KO, Wang P, Zhou C, Duan S (2016) Effect of dimethyl phthalate (DMP) on germination, antioxidant system, and chloroplast ultrastructure in Cucumis sativus L. Environ Sci Pollut Res 23(2):1183–1192CrossRefGoogle Scholar
  57. Zhao HM, Du H, Xiang L, Li YW, Li H, Cai QY et al (2016) Physiological differences in response to di-n-butyl phthalate (DBP) exposure between low-and high-DBP accumulating cultivars of Chinese flowering cabbage (Brassica parachinensis L.). Environ Pollut 208:840–849CrossRefGoogle Scholar
  58. Zhou X, Cui K, Zeng F, Li S, Zeng Z (2016) A simple and selective method for determination of phthalate biomarkers in vegetable samples by high pressure liquid chromatography–electrospray ionization-tandem mass spectrometry. Food Chem 200:336–342CrossRefGoogle Scholar

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